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. 2019 Oct 29;9:15529. doi: 10.1038/s41598-019-52112-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Leucine-rich diet decreased tumour ¹⁸F-fludeoxyglucose (FDG) uptake and reduced number of metastases sites. (A) Graphic showing the standardized uptake values (SUV_max) values; (B) Metabolic tumour volume (MTV; cm³); (C) – Total lesion glycolysis (TLG, SUV [mean] × metabolic tumour volume) in Walker (W/Control) and leucine (LW) tumour-bearing groups; (D) Computed tomography (CT) image of hard tissues analysis; and (E) CT image of soft tissues analysis. The head arrows indicated metastases site. N = 4 animals per group for this analysis procedure. Graphics represent mean ± standard deviation. For details, see the Methods section. *P < 0.05 significance compared to W group (Student’s t-test).