Table 1.
Primer sequences and PCR conditions of studied genes
| Toxin gene | Primers | Oligonucleotide sequences (5' – 3') | PCR conditions | references | |
|---|---|---|---|---|---|
| cdd3 | Time6 Struppi6 |
TCCAATATAATAAATTAGCATTCC GGCTATTACACGTAATCCAGATA |
94°C 5 min, 40 cycles (94°C 1 min; 53°C 1 min;72°C 45 sec), 72°C 5 min | (23, 24) | |
| tcdA | TA1 TA2 |
ATGATAAGGCAACTTCAGTGG TAAGTTCCTCCTGCTCCATCAA |
94°C 5 min, 35 cycles (94°C 1 min; 50°C 1 min;72°C 30 sec), 72°C 5 min | ||
| tcdB | TB1 TB2 |
GACCTGCTTCAATTGGAGAGA GTAACCTACTT CATAACACCAG |
94°C 5 min, 35 cycles (94°C 1 min; 50°C 1 min;72°C 30 sec), 72°C 5 min | ||
| 16S rRNA gene | 27F 1525R |
AGAGTTTGATCCTGGCTCAG AAGGAGGTGWTCCARCC | 94°C 5 min, 40 cycles (94°C 1 min; 60°C 1 min;72°C 45 sec), 72°C 5 min | (25) | |
| C.difficile 16srRNA | C.diff-F C.diff-R | TTGAGCGATTTACTTCGGTAAAGA CCATCCTGTACTGGCTCACCT | 94°C 5 min, 40 cycles (94°C 20 sec; 60°C 1 min, 72°C 5 min), 72°C 5 min | (26) | |