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. 2019 Oct 29;9:15547. doi: 10.1038/s41598-019-52107-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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CKD exhibit normal perfusion recovery and capillary density. (A) Laser Doppler flowmetry measurements of the paw and TA muscles (normalized as a percentage of the contralateral control limb) demonstrated normal perfusion recovery in CKD mice. (D) Total capillary density (labeled by Dylight594-conjugated GS-lectin) was not different between control and CKD mice in either limb muscle. (C,D) Representative IF images of the TA muscle from control and CKD mice labeled with laminin (myofibers), DAPI (nuclei), and GS-lectin (capillaries). N = 5/group for capillary measurements, N = 9/group for perfusion measurements. **P < 0.01 using two-tailed t-test. Error bars represent SEM. FAL = femoral artery ligation.