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. 2019 Oct 29;15:380. doi: 10.1186/s12917-019-2082-7

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — The immune activating effect of anti-CTLA-4 mAb against bovine leukemia virus (BLV) infected cattle PBMCs. PBMCs (n = 10) of BLV-infected cattle were cultured with 20 μg/ml control antibody or anti-CTLA-4 mAb in the presence of fetal lamb kidney cells (FLK)-BLV antigen for 7 days. FLK was used as a control antigen. The IFN-γ production was measured using an ELISA. The bars indicate the average of each group. Statistical comparisons were made using the Steel-Dwass test. The differences were considered statistically significant at p < 0.05