Table 1.
The list of primer used in the experiment
| Primer | Primer sequences (5'-3') | Restriction enzyme |
|---|---|---|
| BLV diagnosis | ||
| BLV-LTR-1 | TGTATGAAAGATCATGCCGAC | |
| BLV-LTR-533 | AATTGTTTGCCGGTCTCT | |
| BLV-LTR-256 | GAGCTCTCTTGCTCCCGAGAC | |
| BLV-LTR-453 | GAAACAAACGCGGGTGCAAGCCAG | |
| β-globin Forward (F) | ACACAACTGTGTTCACTAGC | |
| β-globin Reverse (R ) | CAACTTCATCCACGTTCACC | |
| Establishment of expression vector | ||
| pCXN2.1-CTLA-4-Ig F | CGCGGATATCATGGCTTGCTCTGGATTCCA | EcoRV |
| pCXN2.1-CTLA-4-Ig R | CGGGGTACCATCAGAATCCGGGCATGGTT | KpnI-HF |
| pCXN2.1-CD80-Ig F | CGCGGATATCATGGGTCACACAATGAAGTG | EcoRV |
| pCXN2.1-CD80-Ig R | CGGGGTACCGGTCCAGGTCAGGTGCTGAT | KpnI-HF |
| pCXN2.1-CD86-Ig F | CGCGGATATCATGCGTTTCAAATGCACCAT | EcoRV |
| pCXN2.1-CD86-Ig R | CGGGGTACCTGGGACAGGGGGGCTTGGCA | KpnI-HF |
| pEGFP-N2-CTLA-4 F | GGAAGATCTATGGCTTGCTCTGGATTCCA | BglII |
| pEGFP-N2-CTLA-4 R | CCGGAATTCATTGATGGGAATAAAATAAG | EcoRI |
| pEGFP-N2-CD28 F | GGAAGATCTATGCTCAGGCTGCTCCTGGC | BglII |
| pEGFP-N2-CD28 R | TCCCCCGGGGGAGCGGTAGGCCGCAAAGT | SmaI |
*Recognition sites of restriction enzymes were underlined