Table 1.

Optimized MRM conditions and product ions.

Compound	Transition for quantification Cone (V)/Collision (eV)	Transition for confirmation Cone (V)/Collision (eV)	Reference
Oleacein (3)	319.2 > 195.0 [M-H-C7H8O2]−	319.2 > 165.0 [M-H-C8H10O3]−	6, 22, 23
	8/6	8/8
Oleocanthal (4)	303.2 > 179.0 [M-H-C7H8O2]−	303.2 > 165.0 [M-H-C8H10O2]−	6, 22, 23
	12/6	12/10
Oleuropein aglycone (5)	377.3 > 275.1 [M-H-C4H6O3]−	377.3 > 307.1 [M-H-C4H6O]−	6, 8
	8/14 [M-H-C5H10O2]−	8/10
Ligstroside aglycone (6)	361.3 > 291.1 [M-H-C4H6O]−	361.3> 259.1 [M-H-C4H6O3]−	6, 8
	36/10	36/10 [M-H-C5H10O2]−
Hydroxytyrosol-d5 (1-d5)	158.0 > 126.0 [M-H-CD2O]	158.0 > 97.9	35
	10/14	10/20