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. 2019 Oct 30;14(10):e0223846. doi: 10.1371/journal.pone.0223846

Fig 5. PDGFRβ is required for robust periosteal proliferation in vitro in cells derived from gCblYF mice.

Fig 5

(A) Periosteal cells isolated from PDGFRFL/FL; gCblYF mice were cultured for four days, and then serum starved and infected with AdCre or AdGFP overnight (MOI = 300). Cells were treated with EdU (10mM) for four hours prior to isolation and staining. Flow cytometry analysis of EdU+ periosteal cells in vitro shows significantly less proliferation in periosteal cells following AdCre-mediated deletion of PDGFRβ on the gCblYF background, without PDGF addition. N = 3 *p<0.05 (B) Periosteal cells isolated from PDGFRFL/FL; gCblYF mice were cultured for four days, and then serum starved and infected with AdCre or AdGFP overnight (MOI = 300). Cells were then treated with PDGF-BB and EdU (10mM) for four hours prior to isolation and staining. Flow cytometry analysis of EdU+ periosteal cells in vitro shows significantly less proliferation in periosteal cells treated with PDGF-BB following AdCre-mediated deletion of PDGFRβ on the gCblYF background. N = 3 ***p<0.0005.