Fig 3. Observation of proXTH9::GUS transgenic lines during lateral root (LR) development.

(A–H) GUS staining in proXTH9::GUS Arabidopsis roots at various developmental stages. The seedlings were incubated on media supplemented without (I, J) or with (K, L) 5 mM nitrate for 6 hours. Images of the leaves (I, K) and LRs (J, L) were obtained, and representative images are shown. (M) Relative GUS activity before and after nitrate treatment is shown. Leaf GUS activity in the absence of nitrate was used as a control. The asterisks (*) show statistically significant differences (p<0.05) and the error bars represent SDs (n = 4).