Fig 4. XTH9 gene mutation altered lateral root (LR) development in response to nitrate treatments.
(A–F) Effects of various concentrations of nitrate (10, 50, 100, 500, 1000, 5000, and 10000 μM) on root growth. WT, xth9 mutant, and the complementary line 1 were grown vertically in media supplemented with various nitrate concentrations for 8 days. (G) Primary root length; (H) LR density; (I) Lateral root length were quantified. LR density was calculated by dividing the LR number per 1 cm of primary root. The error bars represent SDs (n = 22–31). Bars with different capital letters indicate significant differences among different concentrations of nitrate treatment in the given genotype by ANOVA analysis. The white bars indicate 1.2 cm.