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. 2019 Oct 18;15(10):e1008465. doi: 10.1371/journal.pgen.1008465

Fig 5. Overexpression of the XTH9 gene increased lateral root (LR) development and improved low-nitrate tolerance.

Fig 5

(A) Root phenotype observation and analysis of (B) LR density, (C) total LR length, and (D) primary root length in WT and XTH9 overexpression lines. Bars with different capital letters indicate significant differences among different nitrate treatment in the given genotype by ANOVA analysis. The white bar indicates 1 cm. (E) Phenotypic analysis of lateral root emergence (LRE) was achieved by synchronizing LR formation with a gravity stimulus for 15 and 36 hours. Compared with the WT (Col-0) plants, the XTH9 overexpression plants showed increased LRE. The data are shown as percentages, and the error bars represent SDs (n = 19–22). The error bars denote SDs (n = 16–23). Approximately 90 total LRP were observed in each plant. (F) WT and transgenic plants were grown vertically in media supplemented with 10 μM nitrate for 8 days. (G) Average LR lengths were quantified. The error bars represent SDs (n = 20–30). Bars with different capital letters indicate significant differences among different concentrations of nitrate treatment in the given genotype by ANOVA analysis. The white bars indicate 1.2 cm.