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Effect of metal ions on serratiopeptidase activity
| Metal ions (5 mM) | Relative activity before chelation and incubation with metal ions (%) | Regaining enzyme activity after chelation with EDTA and incubation with metal ions (%) |
|---|---|---|
| Control | 100 | – |
| Enzyme + EDTA (10 mM) | – | 12 ± 0.8 |
| Ca2+ | 138 ± 1.7 | 68 ± 1.6 |
| Mg2+ | 92 ± 0.9 | 18 ± 1.0 |
| Zn2+ | 109 ± 1.5 | 40 ± 1.4 |
| Cu2+ | 98 ± 1.1 | 19 ± 1.0 |
| Na+ | 105 ± 1.4 | 36 ± 1.3 |
| K+ | 102 ± 1.3 | 27 ± 1.1 |
Each value represents the mean ± SD for three determinations
