Figure 3.

Variants in the rrl gene (23S rRNA) arose during treatment and were present in isolates from concurrent samples. Relative allele fractions at these positions show that, although the initial sputum isolate was susceptible for both patients, resistance appeared to develop during treatment. Samples are ordered by time, with boxes indicating samples taken on the same day. Samples taken on the day of transplant are shown in bold text. Here, following the usual convention for the rrl gene, we use Escherichiacoli numbering. Positions 2058 and 2059 in E. coli correspond to 2269 and 2270 in Mycobacterium abscessus. Abbreviations: BAL, bronchoalveolar lavage; CW, chest wound; DSW, drain site swab; LN, lymph node; LT, lung tissue; PF, pleural fluid; S, sputum.