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. 2019 Sep 2;47(19):10357–10372. doi: 10.1093/nar/gkz747

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© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Pulldown of LYAR and identification of LYAR-associated proteins (A) Silver staining of HBF-LYAR-associated proteins. HBF-LYAR-associated complexes were isolated via sequential two-step pulldown (Ni-NTA pulldown, RNase A treatment, and pulldown of FLAG-tagged HBF-LYAR) from nuclear extract of HBF-LYAR-TO cells or T-REx 293 cells (control) treated with Dox for 24 h. Proteins were subjected to SDS-PAGE and visualized with silver staining. The arrowhead represents HBF-LYAR, as the bait protein. Molecular mass markers (kDa) are indicated to the left. Input: nuclear extract (10 μg). (B) Immunoblotting of HBF-LYAR-associated proteins using antibodies indicated to the right of each panel. 1% Input: 1% of the nuclear extract used for pull down of HBF-LYAR complexes. HBF-LYAR was detected by Stabilized Streptavidin-HRP Conjugate.