Figure 2.

Pol II is differentially bound to promoters of a subset of genes during SynT differentiation. In these studies, ChIP-seq libraries were prepared from CytT isolated from midgestation human placentas before and after SynT differentiation in culture. (a) Numbers of genes that manifested a more than twofold increase (green) or more than twofold decrease (blue) in Pol II binding in SynT vs CytT. (b) RT-qPCR was used to assess expression of selected genes manifesting increased (green) and decreased (blue) binding of endogenous Pol II by ChIP-seq. (c) Representative genes that showed increased Pol II binding in SynT vs CytT. (d) Representative genes that showed decreased Pol II binding in SynT vs CytT. VEGF, vascular endothelial growth factor.