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. 2019 Jul 30;47(19):e114. doi: 10.1093/nar/gkz659

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© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Effects of DIBO, BCN and DIBO-Alexa Fluor-488 conjugation on viral transduction capability. (A) Chemical structures of DIBO, BCN and DIBO-Alexa Fluor-488. (B) Effects of DIBO, BCN and DIBO-Alexa Fluor-488 conjugation on lentiviral vector transduction capability in 293T cells. (C), Effects of DIBO, BCN and DIBO-Alexa Fluor-488 conjugations at residue Y77 or Y116 on lentiviral vector transduction capability in five different cell lines. Viral transduction capability was measured by relative luciferase activity. The expression levels of BCN and DIBO-Alexa 488 were too small to see at this scale. ‘-’ indicates no conjugation as negative control. ***P < 0.01 versus corresponding control, n = 3.