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. 2019 Aug 13;25(10):625–637. doi: 10.1093/molehr/gaz045

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© The Author(s) 2019. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For permissions, please e-mail: journals.permission@oup.com.

This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model)

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Effects of decidualizing hormones and PD98059 (PD) on NESC proteins: Cx43, ERK1/2, p90RSK, ER-α and PR-A/B (western blots). (A) The ERK1/2 inhibitor (PD), hormones (H) or the combination of the two were incubated for 3 (PD + H 3d) or 7 days (PD + H 7d) and their effects on Cx43 (panel 1), phospho-ERK1/2 (panel 2), phospho-p90RSK (panel 3) and β-actin levels (panel 4) were measured. (B) Cx43 (panel 1), ER-α (panel 2), PR-A/B (panel 3) and β-actin levels (panel 4) were also evaluated, relative to control ESC. Molecular weights (kDa) of the specific protein bands are indicated at the right. Full length images of the western blots are available on request.