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. 2019 Aug 13;25(10):625–637. doi: 10.1093/molehr/gaz045

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© The Author(s) 2019. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For permissions, please e-mail: journals.permission@oup.com.

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Effects of IL-1β and hormones (H) on NESC phosphoprotein expression. (A) Kinetics of IL-1β effects on phospho-ERK1/2 (P-ERK1/2, panel 1), phospho-p90RSK (P-p90RSK, panel 2), phospho-Cx43 isoforms (at Ser367 and Ser368 residues; panels 3 and 4), phospho-ER-α (Ser118 and Ser167; panels 5 and 6), phospho-PR-A and -B (Ser294, panel 7) and β-actin (panel 8). (B) Brief exposure (20 or 40 min) to IL-1β reduced total Cx43 (panel 1) but increased phospho-MEK1/2, phospho-ERK1/2, phospho-p90 RSK and phosho-p70/85 S6Kinase (panels 2–5). Phosphorylation of ER-α and PR-A and -B is also shown (panels 6–8). β-Actin effects of decidual hormone treatment for 48 h (H 48 h) are presented in the far right lane. Full length images of the western blots are available on request.