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. 2019 Aug 13;25(10):625–637. doi: 10.1093/molehr/gaz045

Figure 9.

Figure 9

Suppression of MAP kinase signal cascade by PD and effects of IL-1β in NESC and EESC. (A) NESC cultured for 20 min to 72 h with PD were evaluated for phospho-MEK1/2 (panel 1), phospho-ERK1/2 (panel 2), phospho-p90RSK (panel 3), phospho-Cx43 (Ser367 and Ser368 isoforms, panels 4 and 5, respectively), non-phosphorylated Cx43 (Ser368, panel 6) and β-actin (panel 7). Similar findings were noted in two independent ESC preparations. (B) The effects of IL-1β (+) on Cx43 (panel 1), ER-α (panel 2), PR-A and -B (panel 3) and β-actin (panel 4) are shown in four independent preparations each of NESC (1–4) and EESC (1–4). (C) In two additional preparations of EESC (5–6) and NESC (5–6), the effects of IL-1β and PD + IL-1β are compared to the control (Cont) for Cx43 (panel 1), ER-α (panel 2), PR (panel 3) and β-actin (panel 4). Full length images of the western blots are available on request. EESC, eutopic endometrial stromal cells.