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. 2019 Sep 9;47(19):10181–10201. doi: 10.1093/nar/gkz769

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© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 3. — The interaction between SLX4IP and SLX4 is coordinated with XPF. (A) Tandem affinity purification and mass spectrometry (TAP-MS) was conducted to identify SLX4IP-interacting proteins. Lists of high-confidence candidate interacting proteins from mass spectrometry analysis of cells treated with or without mitomycin C (MMC) are presented. (B) HEK293A cells were transfected with constructs encoding SLX4-SFB or its deletion variants and were subjected to immunoprecipitation (IP) with S beads. Western blotting was conducted with antibodies as indicated. (C) Experiments were done as in B, except that SLX4-SFB wild type and SLX4^m-SFB (L530A, F545A, Y546A and L550A) variants were subjected to precipitation with S beads. FL, full length (controls); PSM, peptide spectrum match.