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. 2019 Sep 9;47(19):10296–10312. doi: 10.1093/nar/gkz782

Figure 7.

Figure 7.

The effect of OZM on transcription through polythymidine sequences by Eco RNAP. (A) TECs were assembled using the scaffolds shown above the gel panels (only the non-template DNA strands are shown) and chased with 100 μM ATP, CTP, GTP and UTP or OZM triphosphate or ΨTP, for 5 min at 25°C. The sequences corresponding to the annealing region of the RNA primer are underlined. Thymidines in the transcribed region are highlighted. Pixel counts were linearly scaled to span the full 8-bit grayscale range within each gel panel. Each assay was performed in triplicate. (B) Lane profiles from gels in (A) depicting the effect of GreA on the transcription of four- and seven-thymidine tracts. (C) TECs arrested at the four-thymidine tract (S326 template) were purified by gel-filtration and supplemented with 100 μM NTPs in the absence and presence of 2μM GreA or GreB for 5 min at 25°C.