Figure 1.

CRISPR/Cas9 genome editing strategy to generate mice Mnd1^K85M. (A) Schematic of the human meiotic nuclear divisions 1 (MND1) protein and amino acid and nucleotide sequences surrounding the target single nucleotide polymorphism (SNP) in the human and mouse genomes. Point changes introduced by the single stranded homologous recombination (ssODN/HR) template are indicated as follows: yellow highlighted area contains the altered amino acid (M) in red, and the nucleotide that was mutated text/(underlined) to cause the amino acid change that models the human SNP variant; black text/underline = silent nucleotide changes to prevent Cas9 re-cleavage, including the PAM site (also bolded); blue text = HaeIII restriction enzyme site used for genotyping. K85M, MND1^K85M. (B) Chromatograms of wild-type (WT) and Mnd1^K85M/K85M mice (sequence is reverse complement of the sequences above).