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. 2019 Aug 8;25(10):668–673. doi: 10.1093/molehr/gaz048

Figure 2.

Figure 2

Mnd1K85M/K85M males have normal sperm counts and spermatogenesis, but exhibit sex distortion. (A) Litter sizes from matings of indicated genotypes of males or females to WT mates. WT mice were intercrossed, so no sex is indicated. MUT = Mnd1K85M/K85M. Each point represents a litter (males and females combined), and horizontal bars mark the average and SEM. (B) Progeny separated by sex of WT and Mnd1K85M/K85M (MUT) mice mated to WT animals. Note that mutant males (sires) produce a 2:1 ratio of male:female offspring. The P value is from Student’s t-test. (C) Total sperm quantified from both epididymides of each animal. N = 3–5 per genotype. P values are from Student’s t-test. Het: heterozygous, Homo: homozygous. (D) Relative body:testis masses. N = 4–5 per genotype. P values are from Student’s t-test. (E) Testis histology. Hematoxylin and eosin stained testis cross-sections of 8-week-old WT and mutant males. Highlighted in selected seminiferous tubules of the mutant sample are normal examples of elongated spermatids (arrows), round spermatids (asterisks) and flagellated, spermiated spermatozoa (dashed ellipse in lower right tubule). Size bar = 75 μM.