Figure 7.

RT-qPCR quantification of mRNAs in livers of CV and GF mice exposed to corn oil, PCN, and TCPOBOP (n = 4–5 per group) for prototypical PXR- and CAR-regulated target genes (A), primary BA synthesis (B), hepatic BA uptake transporters (C), basolateral efflux transporters for BAs and cholesterol (D), canalicular efflux transporters for BAs, lipids, and cholesterol (E and F), and the repressive nuclear receptor Shp (G). The mRNA was calculated by dividing the ddCq for the gene of interest by the ddCq for the geometric mean of β-actin and Gapdh. Asterisks (*) represent statistically significant differences as compared with corn oil-exposed group of the same enterotype; pounds (#) represent statistically significant differences between CV and GF mice exposed to the same chemical (two-way ANOVA followed by Tukey’s post hoc test with adjusted p-value < .05). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)