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. 2019 Sep 25;47(19):10452–10463. doi: 10.1093/nar/gkz772

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© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — In vivo enrichment of inducible promoters by fluorescence-activated cell sorting. (A) Cells expressing GFP regulated by engineered promoter variants constitu-tively (top, aTF-/inducer-), co-expressing aTF without inducer (middle, aTF+/inducer-), and co-expressing aTF with inducer (bottom, aTF+/inducer+). Fold repression (FR) is the ratio of median fluorescence of aTF-/inducer- and aTF+/inducer- cells. FR is shown in the middle panel. Fold induction (FI) is the ratio of median fluorescence of aTF+/inducer+ and aTF+/inducer- cells. FI is shown in the bottom panel (B) Cells repressed by aTF (aTF+/inducer-) are sorted into bins according to their fluorescence and each bin is induced independently. Top panel is overall distribution of repressed cells. Colors represent cells sorted into different fluorescence bins. Lower panels show ligand-induced response of cells from each bin. Fold induction (FI) ratio of each bin is mentioned in the panel.