Table 3.

Thermodynamic parameters for wild-type yedK and mutants binding different DNA substrates from ITC experiments

Protein	DNA	N	ΔH (kcal/mol)	ΔS (cal/mol/K)	K d (μM)
WT	ssDNA	1.14	–6.46	4.37	2.2
C2A	ssDNA	1.08	–4.95	6.75	7.9
R4A	ssDNA	0.93	–15.97	–33.2	34.6
P40G	ssDNA	1.26	–8.60	–6.10	10.8
W67A	ssDNA	–	–	–	NB
W68A	ssDNA	–	–	–	NB
K70A	ssDNA	0.82	–5.93	4.81	4.0
N75A	ssDNA	1.36	–5.95	0.28	37.9
R77A	ssDNA	–	–	–	NB
T80A	ssDNA	1.24	–7.05	–1.22	12.6
S84A	ssDNA	0.93	–5.42	4.54	10.8
R85A	ssDNA	1.50	–4.97	5.17	16.9
E105A	ssDNA	1.26	–9.55	–0.43	0.12
W106A	ssDNA	1.48	–9.30	–9.53	18.7
K113A	ssDNA	1.14	–6.46	4.37	2.1
T149A	ssDNA	–	–	–	NB
H160A	ssDNA	1.17	–10.27	–7.68	1.4
R162A	ssDNA	–	–	–	NB
WT	ssDNA_THF	1.19	–14.0	–15.9	0.17
E105A	ssDNA_THF	1.34	–13.60	–13.0	0.07
H160A	ssDNA_THF	1.11	–16.16	–22.0	0.09
WT	5′ junction	–	–	–	NB
WT	3′ junction	0.95	–6.12	10.3	0.18

NB: No binding. Please note that all raw data from the ITC experiments are shown in Supplementary Figure S3A-W.