TableII.
Primers and probes for PCR amplifications.
| Gene/accession number | Forward primer | Reverse primer | FL probe | LC probe | Amplicon (nt) |
|---|---|---|---|---|---|
| Prolactin/ NM_000948 |
gggAAACgAATg CCTgAT |
CAAACAggTCTCg AAgggT |
CCTgCTCCTgTgCC AgAgCgTg-FL |
LC-CCCCCTTgCCC ATCTgTCCCg—PH |
274 |
| Prolactin receptor/ NM_000949 |
TggTTCACgCTCCTgT ATgAA |
TggACTCCATgCA CTCCAgT |
TCAgCCTACATCCAgg ACAgAAATACCTTg—FL |
LC-CCAggTTCgCTg CAAACCAgAC--PH |
174 |
| GAPDH/NM_002046 | gAAggTgAAggTCgg AgTC |
gAAgATggTgATggg ATTTC |
AggggTCATTgATggC AACAATATCCA-FL |
LC-TTTACCAgAgTTAA AAgCAgCCCTggTg-p |
226 |