Figure 10.

Estimated donor and acceptor mean dwell times in the bright photostate from experimental data. Each panel summarizes the posterior probability distributions $\mathcal{P}\left(T^{\text{D}}|{\overrightarrow{I}}_j^{\text{D}},{\overrightarrow{I}}_j^{\text{A}}\right)$ and $\mathcal{P}\left(T^{\text{A}}|{\overrightarrow{I}}_j^{\text{D}},{\overrightarrow{I}}_j^{\text{A}}\right)$ (left and right, respectively), obtained from individual intensity traces ${\overrightarrow{I}}_j^{\text{D}}$ and ${\overrightarrow{I}}_j^{\text{A}}$, for j = 1, …, 100, under increasing concentrations of the oxygen scavenger Trolox. For clarity, in all panels color encodes $\text{log}\mathcal{P}\left(T^{\text{D}}|{\overrightarrow{I}}_j^{\text{D}},{\overrightarrow{I}}_j^{\text{A}}\right)$ and $\text{log}\mathcal{P}\left(T^{\text{A}}|{\overrightarrow{I}}_j^{\text{D}},{\overrightarrow{I}}_j^{\text{A}}\right)$ while vertical axes are shown in logarithmic scale. As can be seen, despite the variability found between individual traces, estimated mean dwell times, from ≈10² frames at zero TQ, increase to ≈10³ frames at 7500 μM Trolox, indicating an approximately 10-fold increase in the mean duration of the photobright periods for either fluorophore.