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. Author manuscript; available in PMC: 2019 Oct 30.
Published in final edited form as: J Phys Chem B. 2019 Jan 10;123(3):675–688. doi: 10.1021/acs.jpcb.8b09752

Figure 4.

Figure 4.

Simulated molecule, mimicking real single molecule experiments, that transitions stochastically between states σ1, σ2, and σ3 (upper panel). As each of these states is associated with different photoemission rates, recorded intensities change over time (middle panel). Accordingly, FRET efficiency, assessed through the recorded intensities, also changes over time in a manner that reflects the underlying state of the molecule (lower panel). Due to blinking, of either the donor or acceptor, measured intensities occasionally drop to background levels. As a result, near 0% or 100% FRET efficiencies are observed, suggesting dwells to artifactual states beyond σ1, σ2, and σ3.