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. 2019 Sep 24;8:e48958. doi: 10.7554/eLife.48958

Figure 5. RNA-seq identifies anterior-enriched transcripts that are expressed predominantly in non-cycling cells.

Figure 5.

(a) WISH of tapeworm anteriors for transcripts that were anterior-enriched (FC ≥1.5, FDR ≤ 0.05) or -depleted (FC ≤−1.5, FDR ≤ 0.05) by RNA-seq. Panels oriented anterior facing left. (b) Differential gene expression analyses of 1 mm anterior, middle, and posterior neck fragments overlaid with irradiation-sensitive transcripts. (c) WISH of transcripts that were anterior-enriched and irradiation-sensitive by RNA-seq that showed expression in a subset of cells in the neck parenchyma. (d) Confocal sections from dFISH of anterior-enriched transcripts (green) and mcm2 (magenta). Cyan arrowheads indicate cells that are magnified at the far right.