Key resources table.
Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
---|---|---|---|---|
Strain, (Hymenolepis diminuta) | BioSample accession SAMN11958994 | Carolina Biologicals | Cat# 132232 | |
Antibody | anti-Oregon Green 488-HRP antibody (rabbit polyclonal) | Invitrogen | A21253 | IF(1:1000) |
Antibody | anti-DIG-AP (sheep polyclonal) | Sigma Aldrich | Cat# 11093274910 | IF(1:2000) |
Antibody | anti-DIG-POD (sheep polyclonal) | Sigma Aldrich | Cat#: 11207733910 | IF(1:2000) |
Antibody | anti-DNP-HRP (rabbit polyclonal) | Vector Laboratories | Cat#: MB-0603 | IF(1:2000) |
Antibody | anti-acetylated α-tubulin (mouse monoclonal) | Santa Cruz | Cat#: sc-23950 | IF(1:500) |
Sequence-based reagent | PCR primers | This paper | Supplementary file 1E | |
Sequence-based reagent | Transcriptome Shotgun Assembly (Hymenolepis diminuta) | DDB/ENA/Genbank | GHNR01000000 | |
Sequence-based reagent | Sequence Read Archives for transcriptome assembly | DDB/ENA/Genbank | PRJNA546290 | SRX6045715- SRX6045719 |
Sequence-based reagent | Sequence Read Archives for differential gene expression | DDB/ENA/Genbank | PRJNA546293 | SRX6064929- SRX6064933 |
Recombinant DNA reagent | Plasmid- pJC53.2 | Addgene | 26536 | |
Chemical compound, drug | F-ara-EdU | Sigma Aldrich | T511293 | 0.1 μM (in 1% final DMSO concentration) |
Chemical compound, drug | Oregon green 488-azide | Invitrogen | O10180 | 100 μM |
Chemical compound, drug | Hydroxyurea | Sigma Aldrich | Cat#: H8627 | 50 mM |