FIGURE 5.

Apolipoprotein B/E (apoB/E) precipitation and HDL isolation. HDLs were isolated from in vivo plasma samples (collected up to 20 min postinfusion) by precipitating apoE- and apoB-containing lipoproteins using magnesium/dextran sulfate (n = 10 per group, except subject 5’s sample was lost for 40% intervention due to technical difficulties). (A) ApoB/apoE precipitation, concentrations (mean ± SEM), d₆-α-T half-life averaged 3.0 min; none of the curve parameters were significantly different between the 2 interventions, F-test P = 0.3826. (B) HDL, concentrations [40% fat, d₆-α-T (nmol/L plasma) = 20.6X + 21.0; 0% fat, d₆-α-T = 21.4X + 20.5; where X = minutes]; neither the slopes nor the y-intercepts were significantly different between the 2 interventions, F-test P = 0.9805. (C) d₆-α-T enrichments in apoB/E and HDL particles. Only 2 samples were obtained for the 0% fat-fast intervention and are shown in C as a circle at 0 and 10 min that overlaps HDL data.