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. Author manuscript; available in PMC: 2020 Dec 1.
Published in final edited form as: J Mol Recognit. 2019 Aug 16;32(12):e2809. doi: 10.1002/jmr.2809

TABLE 1.

DNA aptamer sequences (5’→ 3’) and the BNP amino acid sequence

DNA Aptamer Sequence (5’ → 3’)
Capture Aptamer (25cF) 5’-ATACGGGAGCCAACACCA-CCTCTCACATTATATTGTG AATACTTCGTGCTGTTT-AGAGCAGGTGTGACGGAT-3’
Reporter Aptamer (2F) 5’-ATACGGGAGCCAACACCA-CGTTGCGCAGCTGGGGGC AGTGCTCTTTCGATTTGG-AGAGCAGGTGTGACGGAT-3’
Target Peptide Amino Acid Sequence
BNP (32 amino acids) Ser-Pro-Lys-Met-Val-Gln-Gly-Ser-Gly-Cys-Phe-Gly-Arg-Lys-Met-Asp-Arg-Ile-Ser-Ser-Ser-Ser-Gly-Leu-Gly-Cys-Lys-Val-Leu-Arg-Arg-His

Notes: Eighteen base constant sequence PCR primer ends built into the aptamers are in bolded.