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. 2019 May 10;104(11):2215–2224. doi: 10.3324/haematol.2018.205351

Figure 3.

Figure 3.

Hematopoietic stem cell (HSC) expansion caused by Aml1-ETO is reversed by K-RasG12D. (A) Absolute number of CD45.2 HSC in the bone marrow (BM) from recipients of CON (n=11), AM (n=14), KM (n=13) and AKM fetal liver (FL) cells (n=14). Results were generated in three independent experiments; (B) Representative FACS plots showing gating used to quantify HSC as a percentage of the BM mononuclear cells across all experiments. (C) Percentage reconstitution of total CD45.2 cells, CD45.2 myeloid (LiveCD19−CD4−CD8a−NK1.1−), CD45.2 B cells (LiveNK1.1-Mac1-CD19+) and CD45.2 T cell (LiveNK1.1−Mac1−CD4+CD8a+) compartments in primary, secondary and tertiary transplantations. (D) Absolute number of CD45.2 HSC in secondary recipients of CON (n=9 recipient mice in 2 independent experiments), AM (n=10 recipient mice in 3 independent experiments), KM (n=4 recipient mice in 2 independent experiments), and AKM FL cells (n=7 recipient mice in 2 independent experiments). (E) Replating efficiency of CD45.2 LSK BM cells. Average number of colonies is shown for 5-6 biological replicates per genotype in two independent experiments. The results were analyzed using multiple comparison ANOVA. The results are presented as the mean±Standard Error of Mean. *P<0.05; **P<0.01; ***P<0.001; ****P<0.0001.