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. 2019 Oct 24;10:2443. doi: 10.3389/fmicb.2019.02443

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Copyright © 2019 Quyen, Nordentoft, Vinayaka, Ngo, Engelsmenn, Sun, Madsen, Bang and Wolff.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Optimization of LAMP for reaction volume (A) and amplification time (B). In panel (A), lane 4, 5 and 6: 6, 12.5, and 25 μL reaction volume, respectively. In panel (B), lane 4, 5, 6 and 7: 15 min, 30 min, 45 and 60 min, respectively. In both panels (A,B): lane 1: negative control, lane 2: positive control, and lane 3: 100 bp ladder.