FIGURE 6.

Time of drug addiction studies with C. racemosa, O. obtusiloba, and K. alvarezii crude extracts on CHIKV replication. Vero cells were infected with CHIKV (10⁶ PFU/mL) on MOI of 0.1 following each treatment (before the infection, –3, –2, and –1 h; during the infection, 0h; and after the infection, +1, +2, +4, +8, +12, +16, +20, and +24 h), the results were evaluated by plaque assay. Experiments were performed in triplicate. Ribavirin (5 μM) was used as a positive control. ^∗p < 0.05; ^∗∗p < 0.01 in Dunnett test vs. control (Ribavirin). DMSO used as solvent remained at the final concentration of 0.01% showing no activity on CHIKV replication.