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. 2019 Oct 24;10:1209. doi: 10.3389/fpls.2019.01209

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Copyright © 2019 Betts, Basu, Bolar, Booth, Chang, Cigan, Farrell, Gao, Harkins, Kinney, Lenderts, Li, Liu, McEnany, Mutti, Peterson, Sander, Scelonge, Sopko, Stucker, Wu and Chilcoat

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Measurement of transgene expression levels in transformants generated by site-specific integration (SSI). One to four independent SSI events were characterized at each genomic insertion site. The x-axis labels indicate the chromosome number followed by site number (arbitrary) and ending with a small case letter to identify independent events at the same site (a–d). Error bars on blue and gold bars indicate 95% confidence intervals. Gray bars indicate average ± standard deviation of all events. (A) Concentrations of NPTII protein in leaf extracts from 58 independent maize transformants were measured by mass spectrometry. Results from 840 plants are summarized; each bar is the mean of 10–16 plants. (B) Concentrations of GAT protein in leaf extracts from 17 independent soybean SSI transformants. Results from 167 plants are summarized; each bar is the mean of 9–10 plants.