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. 2019 Oct 24;10:1209. doi: 10.3389/fpls.2019.01209

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Copyright © 2019 Betts, Basu, Bolar, Booth, Chang, Cigan, Farrell, Gao, Harkins, Kinney, Lenderts, Li, Liu, McEnany, Mutti, Peterson, Sander, Scelonge, Sopko, Stucker, Wu and Chilcoat

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of promoter choice and genomic location on transgene expression level. Three transgenes encoding IP2-127 were inserted at five site-specific integration (SSI) landing sites. A set of perfect SSI transformants were recovered and analyzed for IP2-127 protein concentration in young leaf tissue according to the standard protocol. (A). Structure of SSI transformants encoding PMI and a chloroplast targeted IP2-127 protein. The PMI cassette was present in all three SSI structures. (B). IP2-127 protein concentration. Genomic location indicates chromosome number followed by SSI site number. The small case letter identifies a unique SSI landing site transformant. Bars with the same color identify independent excision-exchange transformants at the same SSI landing site (molecularly identical sister transformants). The chart contains data from 365 plants; each bar is the mean of 10-20 plants.