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. 2019 Oct 24;10:1209. doi: 10.3389/fpls.2019.01209

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Copyright © 2019 Betts, Basu, Bolar, Booth, Chang, Cigan, Farrell, Gao, Harkins, Kinney, Lenderts, Li, Liu, McEnany, Mutti, Peterson, Sander, Scelonge, Sopko, Stucker, Wu and Chilcoat

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Grain yield for maize hybrids containing a site-specific integration (SSI) landing site. SSI landing sites at 32 unique genomic locations are each compared to a nontransgenic null segregant derived from the same regenerated parent plant. Inbred pollen donors were T3 generation plants either homozygous or null for the indicated SSI site. Pollen donors were top-crossed to five elite line testers to produce F1 hybrid seed for grain yield testing. Yield trials were carried out in a range of optimal and limited-irrigation conditions in the US Corn Belt in 2015. Each bar represents data from five hybrids at up to 16 locations. Asterisk indicates significant differences in yield between a null segregant and corresponding SSI target line.