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. 2019 Oct 30;9:15629. doi: 10.1038/s41598-019-52239-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Hierarchy of NLS and NES signals in TCF4 (A) Schematic representation of TCF4 protein. Region of studied area of TCF4 is shown by the red rectangle. Expressed deletion mutant of TCF4 is depicted as an arrow. The length of each domain in the diagram is arbitrary. (B) Subcellular localizations of the expressed protein were analysed by confocal microscopy 20-24 h after transfecting COS-7 and N2a cells in the absence or presence of additional factors such as LMB or methanol. Representative images (single confocal plane for confocal microscopy) of subcellular distribution of the TCF4/1-601 area. Bar, 10 µm. Ratios between mean fluorescence intensity in cytoplasmic, nuclear and nucleolar compartments are presented as an accumulated bar graph (no- nucleolus; nu- nucleus, cyt- cytoplasm). (a-c) YFP-TCF4/1-601 in COS-7 cells, (d-f) YFP-TCF4/1-601in N2a cells (g,h) YFP-TCF4/1-601 after methanol addition in COS-7 cells, (i,j) YFP-TCF4/1-601 after methanol addition in N2a cells (k) YFP-TCF4/1-601 after LMB addition in COS-7 cells (l) YFP-TCF4/1-601 after LMB addition in N2a cells.