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. 2019 Oct 30;9:15645. doi: 10.1038/s41598-019-52218-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Wnt5a induces redistribution of Ror2 homodimers and Ror2/dsh heterodimers. The subcellular localization of fluorophore tagged Ror2 homodimers (a) and Ror2/dsh heterodimers (b) was analyzed by a bimolecular fluorescence complementation assay (BiFC) in transiently transfected Xenopus tissue culture cells. The relative grey values show the intensity profiles at the positions marked by yellow bars. (c,d) The membrane/cytoplasm values were calculated in the following way: membrane/cytoplasm = (intensity at the membrane – intensity outside)/(intensity inside – intensity outside). Shown are the mean values and standard errors of at least 399 profiles of more than 60 cells from at least ten independent experiments. Median and dispersion (25–75% range) are shown as box-and-whiskers plots in Supplementary Fig. 5b. (d) The effect of endocytosis inhibitors chlorpromazine and genistein on the subcellular distribution of Ror2/dsh heterodimers. Shown are the mean values and standard errors of at least 280 profiles of more than 40 cells in at least seven independent experiments. p values according to Student’s t-test, n.s.: not significant. Median and dispersion are shown in Supplementary Fig. 5c.