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. 2019 Oct 30;9:15645. doi: 10.1038/s41598-019-52218-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Caveolin regulates internalization of Wnt5a/Ror2 clusters. (a) Colocalization of Ror2mCherry/Wnt5aeGFP clusters in animal cap explants of caveolin1 and caveolin3 morphants and caveolin1 and caveolin3 overexpressing embryos. (b,c) Quantification of the clusters. More than 80% of the clusters are located at the plasma membrane. Overexpression of caveolin1 slightly increases the frequency of membrane localization. (c) Average number of intracellular clusters per cell. p values according to Students t-test. (d) The BiFC signal of Ror2/dsh dimers partially colocalizes with the red fluorescence of caveolin1mGarnet in transfected XTC-cells, both, at the plasma membrane and in the cytoplasm (arrowheads in the close-ups). (e) Single particle tracking using widefield fluorescence microscopy indicates that cav1eGFP (blue trajectory) and Ror2mcherry (red trajectory) co-migrate in the cytoplasm (arrowheads) and leave the membrane together and continue co-migrating in the cytoplasm (arrows).