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. 2019 Oct 30;9:15642. doi: 10.1038/s41598-019-52194-6

Figure 5.

Figure 5

The recovery of the Cav3.3 current is abolished in the presence of sodium azide. (A,B) Examples of Cav3.3 currents elicited by the 1 Hz stimulation protocol during 40 s for the 1st and the 3rd stimulation in the control condition, Ctrl (A), and in cells treated with sodium azide, NaN3 10 mM for 2 hours (B). (C) Amplitude of the Cav3.3 current at the beginning (1 s) and after 40 s obtained at the 1st and the 3rd stimulation normalized to the initial current amplitude I (1 s 1st). (D) Inactivation kinetics of the Cav3.3 current at the beginning of the stimulation (1 s) and after 40 s stimulation obtained for the 1st and the 3rd stimulation. Asterisks indicate significant difference in the current amplitude (C) and inactivation kinetics (D), as compared to the initial current. In addition, variation in the current amplitude (C) and in inactivation kinetics (D) as function of the NaN3 treatment was statistically compared to the respective control condition, as indicated. The number of cells tested is indicated into brackets. (n.s.: non-significant).