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. 2019 Oct 24;10:1268. doi: 10.3389/fphar.2019.01268

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Copyright © 2019 Huang, Zhang, Qi, Shi, Song, Liu and Sun

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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FoxO4 was the direct target of miR-499 in myocardial infarction in vitro. (A) Sequence alignment show between miR-499 and the binding sites in the 3’UTR of the FoxO4 gene. (B) The interaction between miR-499 and its binding sites in the 3’UTR of FoxO4 was examined by luciferase assay in HEK293 cells. (C) The expressions of FoxO4 protein were measured in vitro. (D) The expressions of FoxO4 protein were measured in myocardial infarction. B&C: **P < 0.01 versus control, ^## P < 0.01 versus miR-499 mimic; D: **P < 0.01 versus sham. n = 4.