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. 2019 Oct 24;10:1334. doi: 10.3389/fpls.2019.01334

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Copyright © 2019 Desiderio, Salzano, Scaloni, Massa, Pimpinella, De Coste, Pioli, Nardi, Benvenuto and Villani

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunoblotting analysis performed to validate 2D-DIGE results. (A) Specific polyclonal antibodies were used to detect the expression of enolase and ATP synthase in HRCs, independently exposed to X- and γ-radiation. Actin expression was used to normalize protein quantity. (B) Immunoblot signals were quantified by densitometric analysis. Data are presented as means values obtained from three biological replicates ± standard deviation. Tables show the results of one-way ANOVA followed by Fisher’s least significant difference (LSD) test, based on three replication sample dimension. Statistical significance is indicated with asterisks: ∗ = p < 0.05; ∗∗ = p < 0.01; ∗∗∗ = p < 0.001. ns: no statistically significant differences.