Table 1.

Two-dimensional difference in-gel electrophoresis (2D-DIGE) experimental design.

Gel number	Cy2 internal standard	Cy3 sample	Cy5 sample
1	50 µg (4.17 µg each of samples)	50 µg sample C (1)	50 µg sample 0.5 Gy (1)
2	50 µg (4.17 µg each of samples)	50 µg sample 5 Gy (1)	50 µg sample C (2)
3	50 µg (4.17 µg each of samples)	50 µg sample C (3)	50 µg sample 10 Gy (1)
4	50 µg (4.17 µg each of samples)	50 µg sample 0.5 Gy (2)	50 µg sample 5 Gy (3)
5	50 µg (4.17 µg each of samples)	50 µg sample 5 Gy (2)	50 µg sample 10 Gy (3)
6	50 µg (4.17 µg each of samples)	50 µg sample 10 Gy (2)	50 µg sample 0.5 Gy(3)

Three biological replicates of protein samples (indicated by numbers in parenthesis) were analyzed for each condition (unexposed control C; 0.5, 5, and 10 Gy exposed). The same experimental design was adopted for “hairy root” cultures (HRCs) exposed both to γ- and X-rays.