Fig. 1.

E2F1 acetylation creates a binding motif for the bromodomains of p300 and CBP. a A tri-acetylated E2F1 peptide (3KAc) but not unacetylated peptide (UnAc) binds to recombinant p300 containing the bromo, ring finger, and PHD domains (BRP) in a protein domain microarray. NS indicates nonspecific interaction. b, c E2F1 peptides unacetylated, tri-acetylated, or mono-acetylated at K117, K120, or K125 were incubated with purified GST-fusion proteins containing the bromodomain domain of p300 (b) or CBP (c). Following pull-down, western blot for GST was performed to examine binding between p300 or CBP and the E2F1 peptides. d A ¹H, ¹⁵N heteronuclear single quantum coherence NMR titration experiment was performed using the p300 bromodomain and an acetylated E2F1 peptide. Source data of b and c are provided as Supplementary Data 5