Fig. 3.
Seeded aggregation of α-synuclein in presence of DJ-1Pox. a Increase of relative aggregate mass upon addition of free monomers (50 µM) of α-synuclein to existing seeds (0.1, 1, 5, and 10%) was measured over time by Thioflavin T fluorescence assay. b Elongation rate of α-synuclein seeds in presence of α-synuclein monomers as a function of DJ-1Pox. From three biologically independent experiments. **P, ***P, and ****P < 0.1, analyzed using one-way ANOVA with Bonferroni’s post-hoc test. c AFM images showing seed elongation and inhibition in presence of DJ-1Pox at different time points. Scale bar represents 500 nm