Fig. 8.
Different degrees of neurotoxicity induced by α-synuclein variants in presence and absence of DJ-1Pox SH-SY5Y cells were treated with 10 µM of different α-synuclein oligomers and monomers for 48 h. Cells were then detached using 1X PBS and 5 mM EDTA and apoptosis assay was performed using Annexin V-FITC Apoptosis Detection Kit (Sigma, USA). Different samples (1 × 106 cells/ml) were treated with Annexin V- FITC (5 µl) and PI (10 µl) and incubated at room temperature for exactly 10 min in dark. Labelled cells were sorted by fluorescence using a BD FACS verse Flow Cytometer (BD Biosciences) with a minimum of 10,000 events recorded per sample using BD Cell Quest Pro software. Three biologically independent experiments were performed. Statistical significance was analyzed using one-way ANOVA with Bonferroni’s post hoc test (**P, ***P, and ****P < 0.1). Panel represents percentage of a viable cells, b early apoptotic cells, c late apoptotic cells and d necrotic cells