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. 2019 Oct 30;9:15690. doi: 10.1038/s41598-019-51853-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Negative ion mode DESI-MS imaging data acquired from endometriosis and eutopic endometrium tissues obtained from different patients. (a) Selected DESI-MS profiles from the endometrial glands within ectopic endometrial tissue collected from endometriosis lesions (top) and eutopic endometrial tissue from inside the uterus (bottom). The spectra shown are an average of 10 scans. (b) Selected DESI-MS ion images of endometriosis and endometrium tissues. Regions of endometrial glands and stroma within the lesions are outlined in black on the optical images of the H&E stained tissue sections. (c) High magnification view of an endometriosis tissue showing outlined regions of endometrial glands and stroma that spatially correlate to the distributions of various molecular ions detected by DESI-MS imaging, as exemplified by m/z 303. 233 and m/z 723.478 shown.