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. 2019 Oct 30;9:15690. doi: 10.1038/s41598-019-51853-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Intra-patient analysis of eutopic endometrium and four endometriosis tissues collected from ovary, rectal, bladder and endometrioma (patient #98). (a) Selected DESI mass spectra obtained from three samples, including endometriosis from both the right ovary and an ovarian cyst (endometrioma), and eutopic endometrium. (b) PCA score plots of the per-pixel data extracted from all endometriosis tissues (yellow) versus eutopic endometrium tissue (black). (c) PCA results of the per-pixel data extracted from endometriosis tissues per region of excision (ovary in orange; rectal in pink, bladder in green, endometrioma in blue) versus eutopic endometrium tissue (black). Ellipses are calculated by a one-sigma ellipse (68% probability) of an estimated bivariate Gaussian distribution for each group.